Ganguly K, Kalyanaraman V S, Sarngadharan M G
Cancer Lett. 1983 Feb;18(1):79-86. doi: 10.1016/0304-3835(83)90120-9.
Binding of 125I-labeled gp70 of Rauscher murine leukemia virus (R-MuLV) by 3 murine cell lines, BALB/c-3T3, NIH/3T3 and KA-31 (Kirsten murine sarcoma virus transformed clone A-31 of BALB/c-3T3) cells was measured. The binding was a saturable process, dependent on the concentration of gp70 and on the number of cells. In no experiment could we demonstrate any quantitative utilization of gp70 in the medium. However, gp70 remaining in the spent medium could be bound to fresh cells in a subsequent incubation. BALB/c-3T3, NIH/3T3 and KA-31 cells showed similar association constants (1.2-2.5 x 10(8) M-1) for the binding. Moreover, all 3 cell lines had similar number of receptors (7.4-8.9 x 10(5)) per cell. Neither N- and B-tropism of the cells nor transformation by a sarcoma virus altered the number and type of the cell surface receptors.
检测了劳氏鼠白血病病毒（R-MuLV）的125I标记的gp70与3种鼠细胞系，即BALB/c-3T3、NIH/3T3和KA-31（BALB/c-3T3的 Kirsten 鼠肉瘤病毒转化克隆A-31）细胞的结合情况。这种结合是一个可饱和的过程，取决于gp70的浓度和细胞数量。在任何实验中，我们都无法证明培养基中的gp70有任何定量利用情况。然而，用过的培养基中剩余的gp70在后续孵育中可与新鲜细胞结合。BALB/c-3T3、NIH/3T3和KA-31细胞对这种结合显示出相似的缔合常数（1.2 - 2.5×10⁸ M⁻¹）。此外，所有这3种细胞系每个细胞具有相似数量的受体（7.4 - 8.9×10⁵）。细胞的N向性和B向性以及肉瘤病毒转化均未改变细胞表面受体的数量和类型。
