嗜亲性（莫洛尼）鼠白血病逆转录病毒与NIH 3T3细胞的结合动力学
Binding kinetics of ecotropic (Moloney) murine leukemia retrovirus with NIH 3T3 cells.
作者信息
Yu H, Soong N, Anderson W F
机构信息
Gene Therapy Laboratories, Norris Cancer Center, University of Southern California School of Medicine, Los Angeles 90033, USA.
出版信息
J Virol. 1995 Oct;69(10):6557-62. doi: 10.1128/JVI.69.10.6557-6562.1995.
Abstract
A quantitative analysis of the binding kinetics of intact Moloney murine leukemia retrovirus (MoMuLV) particles with NIH 3T3 cells was performed with an immunofluorescence flow cytometry assay. The virus-cell binding equilibrium dissociation constant (KD), expressed in terms of virus particle concentration, was measured to be 8.5 (+/- 6.4) x 10(-12) M at 4 degrees C and was three- to sixfold lower at temperatures above 15 degrees C. The KD of virus binding is about 1,000-fold lower than the KD of purified MoMuLV envelope. The association rate constant was determined to be 2.5 (+/- 0.9) x 10(9) M-1 min-1 at 4 degrees C and was 5- to 10-fold higher at temperatures above 15 degrees C. The apparent dissociation rate constant at 4 degrees C was 1.1 (+/- 0.4) x 10(-3) min-1 and was doubled for every 10 degrees C increase in temperature over the range tested (15 to 37 degrees C).
摘要
采用免疫荧光流式细胞术对完整的莫洛尼鼠白血病病毒（MoMuLV）颗粒与NIH 3T3细胞的结合动力学进行了定量分析。以病毒颗粒浓度表示的病毒-细胞结合平衡解离常数（KD）在4℃时测得为8.5（±6.4）×10⁻¹² M，在15℃以上温度时低三至六倍。病毒结合的KD比纯化的MoMuLV包膜的KD低约1000倍。结合速率常数在4℃时测定为2.5（±0.9）×10⁹ M⁻¹ min⁻¹，在15℃以上温度时高5至10倍。4℃时的表观解离速率常数为1.1（±0.4）×10⁻³ min⁻¹，在所测试的温度范围（15至37℃）内，温度每升高10℃，该常数就增加一倍。
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