Keltjens J T, Huberts M J, Laarhoven W H, Vogels G D
Eur J Biochem. 1983 Feb 15;130(3):537-44. doi: 10.1111/j.1432-1033.1983.tb07183.x.
During short-time labeling experiments, cells of Methanobacterium thermoautotrophicum incorporate a substantial part of 14CO2 in a yellow fluorescent compound (called YFC) [Daniels, L. & Zeikus, J. G. (1978) J. Bacteriol. 136, 75-84]. As the compound was present only in small amounts, its more abundant, metabolic precursor was identified, extracted and purified by column chromatography. The chromophore of this compound is 2-amino-4-hydroxypteridine (pterin) as indicated by its ultraviolet-visible-light absorption and fluorescence properties. Decomposition studies revealed the presence of a number of structural elements, viz. glutamic acid, phosphate and a hexosamine. 1H-NMR and 13C-NMR spectra pointed to the presence of additional, as yet unidentified, elements. The compound is a complex, novel pterin derivative, which we have called methanopterin.
在短期标记实验中，嗜热自养甲烷杆菌的细胞将大量的(^{14}CO_2)掺入一种黄色荧光化合物（称为YFC）中[丹尼尔斯，L.和泽库斯，J.G.（1978年）《细菌学杂志》136卷，75 - 84页]。由于该化合物含量极少，其含量更丰富的代谢前体通过柱色谱法进行了鉴定、提取和纯化。根据其紫外 - 可见光吸收和荧光特性表明，该化合物的发色团是2 - 氨基 - 4 - 羟基蝶啶（蝶呤）。分解研究揭示了一些结构元素的存在，即谷氨酸、磷酸盐和一种己糖胺。(^{1}H - NMR)和(^{13}C - NMR)光谱表明还存在其他尚未鉴定的元素。该化合物是一种复杂的新型蝶呤衍生物，我们将其称为甲烷蝶呤。
