Sane R T, Doshi V J, Joshi S K
J Assoc Off Anal Chem. 1983 Jan;66(1):158-60.
A simple colorimetric method is described for the determination of pyridoxine hydrochloride (vitamin B6). The method is based on the measurement of an orange species formed when pyridoxine hydrochloride is treated with diazotized dapsone and sulfanilamide in a mixture of trichloroacetic acid and sulfuric acid at room temperature, followed by treatment with an aqueous solution of sodium carbonate. Compounds such as thiamine hydrochloride, cyanocobalamin, and common excipients such as starch and talc which are present in various formulations with pyridoxine hydrochloride do not interfere in the reaction. Statistical validation showed that the method was highly precise and accurate. Results agree well with those obtained by other methods reported in the literature.
描述了一种用于测定盐酸吡哆醇（维生素B6）的简单比色法。该方法基于在室温下，将盐酸吡哆醇与重氮化的氨苯砜和磺胺在三氯乙酸和硫酸的混合物中处理后，形成橙色物质，然后用碳酸钠水溶液处理，通过测量该橙色物质来进行测定。盐酸硫胺素、氰钴胺素等化合物以及盐酸吡哆醇各种制剂中存在的淀粉和滑石粉等常见辅料不干扰该反应。统计验证表明该方法具有很高的精密度和准确性。结果与文献中报道的其他方法所得结果吻合良好。
