Schreurs V V, Boekholt H A, Koopmanschap R E
J Chromatogr. 1983 Jan 7;254:203-10. doi: 10.1016/s0021-9673(01)88334-5.
Gel filtration has been applied to muscle proteins in the presence of the detergent sodium dodecyl sulphate (SDS). This detergent not only solubilizes a variety of proteins but also increases their hydrodynamic volumes, which is a disadvantage for the process of gel filtration. Nevertheless, appropriate choice of gel type, in this case Sephacryl S-400 Superfine, and optimal elution conditions make SDS gel filtration a useful tool in the separation of heterogeneous protein mixtures in the molecular weight range of 20,000-200,000.
凝胶过滤已应用于在去污剂十二烷基硫酸钠（SDS）存在下的肌肉蛋白质。这种去污剂不仅能溶解多种蛋白质，还会增加它们的流体动力学体积，这对凝胶过滤过程来说是个不利因素。然而，合适的凝胶类型（在这种情况下是Sephacryl S - 400 Superfine）的选择以及最佳洗脱条件使SDS凝胶过滤成为分离分子量在20,000 - 200,000范围内的异质蛋白质混合物的有用工具。
