Van de Voorde A, De Broe M E, Pollet D E, Rutsaert R J, Nouwen E J
Biochem Biophys Res Commun. 1983 Mar 29;111(3):1015-21. doi: 10.1016/0006-291x(83)91401-8.
In patients with essential hypertension, an additional plasma protein with an apparent molecular weight of 15,000 has been observed by high resolution sodium dodecyl sulphate polyacrylamide gel electrophoresis in the presence of beta-mercaptoethanol. Gel filtration on Sephacryl S-300 superfine revealed a native molecule with a molecular weight of 105,000. After reduction of the disulfide bridges with beta-mercaptoethanol the native molecule is dissociated into two subunits of 45,000 and one of 15,000. The native plasma protein has been purified by a combination of gel filtration on Sephacryl S-300, affinity chromatography on Concanavalin A Sepharose and anion exchange chromatography on DEAE-Sepharose. This protein has been referred to as "hypertension associated protein" (HAP).
在原发性高血压患者中，通过在β-巯基乙醇存在下的高分辨率十二烷基硫酸钠聚丙烯酰胺凝胶电泳，观察到一种表观分子量为15,000的额外血浆蛋白。在Sephacryl S - 300超细凝胶过滤柱上进行凝胶过滤，显示该天然分子的分子量为105,000。用β-巯基乙醇还原二硫键后，天然分子解离成两个45,000的亚基和一个15,000的亚基。该天然血浆蛋白已通过Sephacryl S - 300凝胶过滤、伴刀豆球蛋白A琼脂糖亲和层析和DEAE - 琼脂糖阴离子交换层析相结合的方法进行了纯化。这种蛋白质被称为“高血压相关蛋白”（HAP）。
