采用免疫组织化学方法检测大鼠眼部对内源性白蛋白和免疫球蛋白G（IgG）的血管通透性。

Vascular permeability in the rat eye to endogenous albumin and immunoglobulin G (IgG) examined by immunohistochemical methods.

作者信息

Pino R M, Thouron C L

出版信息

J Histochem Cytochem. 1983 Mar;31(3):411-6. doi: 10.1177/31.3.6827079.

DOI:10.1177/31.3.6827079

Abstract

Vascular permeability in the rat retina and choroid was examined by localizing endogenous albumin (radius, 35 A) and immunoglobulin G (IgG; radius, 55 A) by immunohistochemistry. Three techniques were used: protein A-horseradish peroxidase, peroxidase-antiperoxidase, and avidin-horseradish peroxidase. The protein A-horseradish peroxidase method yielded the least amount of tissue background staining with a high degree of reaction product found in blood vessels. With this method albumin was identified in retinal capillaries, the choriocapillaris, larger choroidal vessels, and in the stroma of the choroid. Very low levels were found in Bruch's membrane. Reaction product due to IgG was also present intravascularly, but little reaction product was present around the large vessels of the choroid and none was identified in Bruch's membrane. Comparisons were made between these localizations and those of intravenously injected hemeprotein tracers of similar size.

摘要

通过免疫组织化学定位内源性白蛋白（半径35 Å）和免疫球蛋白G（IgG；半径55 Å），研究大鼠视网膜和脉络膜的血管通透性。使用了三种技术：蛋白A-辣根过氧化物酶、过氧化物酶-抗过氧化物酶和抗生物素蛋白-辣根过氧化物酶。蛋白A-辣根过氧化物酶法产生的组织背景染色最少，在血管中发现了高度的反应产物。用这种方法在视网膜毛细血管、脉络膜毛细血管、较大的脉络膜血管和脉络膜基质中鉴定出白蛋白。在布鲁赫膜中发现的水平非常低。由于IgG产生的反应产物也存在于血管内，但在脉络膜大血管周围几乎没有反应产物，在布鲁赫膜中未发现。对这些定位与静脉注射的类似大小的血红素蛋白示踪剂的定位进行了比较。