Bernaudin J F, Bellon B, Pinchon M C, Kuhn J, Druet P, Bignon J
Am Rev Respir Dis. 1982 Jun;125(6):734-9. doi: 10.1164/arrd.1982.125.6.734.
The permeability of the blood-air barrier to antiperoxidase (HRP) IgG antibodies (160,000 daltons), F(ab')2 fragments (100,000 daltons) and Fab fragments (50,000 daltons) was studied in the normal rat. The use of isologous and heterologous immunoglobulin G or IgG fragments injected intravenously allowed a sequential study. It was shown that these proteins transfer from the vascular bed towards to interstitium. Evidence was obtained that these proteins crossed the endothelium through interendothelial spaces or structures suggesting transendothelial channels. The alveolar epithelium was found to be an efficient barrier for heterologous and isologous proteins, raising the question of the origin of serum proteins found in the alveolar surface material. Heterologous anti-HRP IgG antibodies and their Fab fragments were also administered intraalveolarly. The alveolar epithelium was found permeable to Fab fragments but not to IgG molecules, suggesting that some serum proteins present in the alveolar lining fluid can be transepithelially reabsorbed.
在正常大鼠中研究了气血屏障对抗过氧化物酶（HRP）IgG抗体（160,000道尔顿）、F(ab')2片段（100,000道尔顿）和Fab片段（50,000道尔顿）的通透性。通过静脉注射同源和异源免疫球蛋白G或IgG片段进行了一系列研究。结果表明，这些蛋白质从血管床向间质转移。有证据表明，这些蛋白质通过内皮间隙或提示存在跨内皮通道的结构穿过内皮。发现肺泡上皮对异源和同源蛋白质是一种有效的屏障，这就引发了肺泡表面物质中血清蛋白来源的问题。还向肺泡内注射了异源抗HRP IgG抗体及其Fab片段。发现肺泡上皮对Fab片段具有通透性，但对IgG分子不通透，这表明肺泡内衬液中存在的一些血清蛋白可以通过上皮细胞重吸收。
