人免疫球蛋白G、免疫球蛋白A和免疫球蛋白M的放射免疫测定

Radioimmunoassay of human IgG, IgA and IgM.

作者信息

Cripps A W, Virgin R J, Lewins E G, Clancy R L

出版信息

J Immunol Methods. 1983 Feb 25;57(1-3):185-95. doi: 10.1016/0022-1759(83)90077-7.

Abstract

Radioimmunoassay procedures for the quantitation of nanogram quantities of human immunoglobulin are described. The techniques have been successfully used to measure immunoglobulin secretion in culture supernatants by cultured human lymphocytes. Compared with previously published assays to assess lymphocyte function in vitro these procedures are simple, quick and reliable. A comparison of double-antibody and solid-phase radioimmunoassay is made. Similar sensitivity and variability in IgG and IgA assays were observed but it was not possible to develop a reliable double antibody radioimmunoassay for IgM. The solid-phase assay has several advantages over the double-antibody radioimmunoassays being quick to perform and using standard commercial reagents without necessity for exhaustive absorption.

摘要

本文描述了用于定量纳克级人免疫球蛋白的放射免疫分析方法。这些技术已成功用于测量培养的人淋巴细胞在培养上清液中的免疫球蛋白分泌。与先前发表的用于体外评估淋巴细胞功能的分析方法相比，这些方法简单、快速且可靠。本文对双抗体放射免疫分析和固相放射免疫分析进行了比较。在IgG和IgA分析中观察到了相似的灵敏度和变异性，但无法开发出可靠的针对IgM的双抗体放射免疫分析方法。固相分析相对于双抗体放射免疫分析具有多个优点，它操作快速，使用标准商业试剂，无需进行彻底的吸收处理。