Raizada M K, Phillips M I, Gerndt J S
Neuroendocrinology. 1983;36(1):64-7. doi: 10.1159/000123438.
Primary cultures from fetal rat brain contain angiotensin II immunoreactivity in neurons. To study the origin of this immunoreactivity, primary cultures were incubated with [3H]-valine or [3H]-isoleucine. A time-dependent incorporation of radioactivity into immunoprecipitable angiotensin II was observed. Incubation of cultures with Captopril, an inhibitor of the converting enzyme, resulted in a significant inhibition of [3H]-valine incorporation into immunoreactive [3H]-angiotensin II. These results demonstrate that primary cultures from fetal rat brain can synthesize an angiotensin II-like immunoreactivity and support a concept that angiotensin II may be synthesized in fetal rat brain.
来自胎鼠脑的原代培养物中，神经元含有血管紧张素II免疫反应性。为了研究这种免疫反应性的起源，将原代培养物与[3H]-缬氨酸或[3H]-异亮氨酸一起孵育。观察到放射性随时间掺入可免疫沉淀的血管紧张素II中。用转化酶抑制剂卡托普利孵育培养物，可显著抑制[3H]-缬氨酸掺入免疫反应性[3H]-血管紧张素II中。这些结果表明，来自胎鼠脑的原代培养物能够合成一种血管紧张素II样免疫反应性，并支持血管紧张素II可能在胎鼠脑中合成的观点。
