Raizada M K
Exp Cell Res. 1983 Feb;143(2):351-7. doi: 10.1016/0014-4827(83)90061-7.
Brains from 1-day-old rats were dissociated with trypsin and the cells were maintained in culture for 3-4 weeks. These primary cultures contained insulin-like immunoreactivity in the limited populations of neurons. Typically, the fluorescent staining pattern observed in the soma was homogeneous and varicosity-like structures were observed on the neurites of the majority of insulin-like immunoreactive neurons. Serum deprivation of brain cell cultures did not reduce immunoreactivity, whereas cycloheximide caused approx. 80% decrease in the number of insulin-like immunoreactive neurons. Incubation of these cultures with [3H]valine resulted in the incorporation of radioactivity into immunoprecipitable insulin. These results suggest that insulin-like immunoreactivity present in the Central Nervous System (CNS) neurons may be synthesized by brain cell cultures.
将1日龄大鼠的大脑用胰蛋白酶解离，细胞在培养中维持3 - 4周。这些原代培养物在有限的神经元群体中含有胰岛素样免疫反应性。通常，在胞体中观察到的荧光染色模式是均匀的，并且在大多数胰岛素样免疫反应性神经元的神经突上观察到曲张样结构。脑细胞培养物的血清剥夺并未降低免疫反应性，而环己酰亚胺导致胰岛素样免疫反应性神经元数量减少约80%。用[³H]缬氨酸孵育这些培养物导致放射性掺入可免疫沉淀的胰岛素中。这些结果表明，中枢神经系统（CNS）神经元中存在的胰岛素样免疫反应性可能由脑细胞培养物合成。
