利用高效液相色谱法体外研究大鼠空肠对嘌呤的吸收与代谢。

Use of high performance liquid chromatography to study absorption and metabolism of purines by rat jejunum in vitro.

作者信息

Parsons D S, Shaw M I

出版信息

Q J Exp Physiol. 1983 Jan;68(1):53-67. doi: 10.1113/expphysiol.1983.sp002702.

DOI:10.1113/expphysiol.1983.sp002702

Abstract

A simple and specific analytical method incorporating high performance (anion-exchange) liquid chromatography has been used to study the absorption and metabolism of some purine derivatives in an in vitro preparation of adult rat jejunum. When present in the lumen the purine bases guanine (4 X 10(-5)M), hypoxanthine or xanthine (10(-4)-3 X 10(-4)M) do not appear in the serosal secretions but the uric acid (UA) content of these secretions is increased. With UA in the intestinal lumen (10(-4)-3 X 10(-4)M) the serosal UA is increased, in some cases to a higher concentration than that in the lumen. With adenine in the lumen (10(-4)-10(-3)M) there is an increased appearance of UA and adenine also appears in the serosal secretions, but the concentration of adenine never exceeds that in the lumen. It is concluded that purines absorbed from the lumen are significantly metabolized to UA during translocation across rat jejunum. Negligible amounts of metabolites are found in the luminal solutions except for guanine which appears to be degraded in the lumen.

摘要

一种结合高效（阴离子交换）液相色谱的简单且特异的分析方法已被用于研究成年大鼠空肠体外制剂中一些嘌呤衍生物的吸收和代谢。当嘌呤碱鸟嘌呤（4×10⁻⁵M）、次黄嘌呤或黄嘌呤（10⁻⁴ - 3×10⁻⁴M）存在于肠腔时，它们不会出现在浆膜分泌物中，但这些分泌物中的尿酸（UA）含量会增加。当肠腔中有尿酸（10⁻⁴ - 3×10⁻⁴M）时，浆膜尿酸会增加，在某些情况下其浓度高于肠腔中的浓度。当肠腔中有腺嘌呤（10⁻⁴ - 10⁻³M）时，尿酸的出现增加，腺嘌呤也会出现在浆膜分泌物中，但腺嘌呤的浓度从未超过肠腔中的浓度。得出的结论是，从肠腔吸收的嘌呤在穿过大鼠空肠的转运过程中会显著代谢为尿酸。除了鸟嘌呤似乎在肠腔中被降解外，在肠腔溶液中发现的代谢物量可忽略不计。