Rothschild K J, Cantore W A, Marrero H
Science. 1983 Mar 18;219(4590):1333-5. doi: 10.1126/science.6828860.
The membrane protein rhodopsin is the primary light receptor in vision. Fourier transform infrared difference spectroscopy is sensitive to conformational changes in both the protein and the retinylidene chromophore of rhodopsin. By blocking rhodopsin bleaching at specific intermediates, it is possible to elucidate some of the primary molecular events of vision.
膜蛋白视紫红质是视觉中的主要光感受器。傅里叶变换红外差光谱对视紫红质的蛋白质和视黄醛发色团的构象变化都很敏感。通过在特定中间体处阻断视紫红质的漂白,可以阐明一些视觉的主要分子事件。