Hamasaki N, Hirota-Chigita C
Transfusion. 1983 Jan-Feb;23(1):1-7. doi: 10.1046/j.1537-2995.1983.23183147295.x.
Stored, depleted RBC were rejuvenated with respect to their levels of adenosine triphosphate (ATP), 2,3-diphosphoglycerate (2,3-DPG), and P50 by acid-citrate-dextrose perservatives containing phosphoenolpyruvate (PEP) without sucrose. The restorations of P50 and 2,3-DPG were dependent on the phosphoenolpyruvate concentration. Erythrocyte P50 and 2,3-DPG, even after treatment with these preservatives, decreased with increasing storage period, but the P50 and 2,3-DPG of five-week-old blood were still higher than the corresponding values of fresh blood. ATP concentration was also increased by treating stored blood with preservatives containing phosphoenolpyruvate, but the elevated ATP of five-week-old blood was only about 50 percent of fresh blood. The ATP level could not be raised further by increasing phosphoenolpyruvate concentration but was improved by supplementation with adenine and nucleosides. Incubation of stored blood with 15 mM phosphoenolpyruvate was sufficient to restore ATP, 2,3-DPG and P50 of three-week-old blood to nearly normal. The results of these studies indicate that sucrose is not necessary for PEP to be effective as a preservative additive.
储存的、已消耗的红细胞，通过不含蔗糖但含有磷酸烯醇丙酮酸（PEP）的酸性枸橼酸盐葡萄糖防腐剂，在三磷酸腺苷（ATP）、2,3 - 二磷酸甘油酸（2,3 - DPG）水平及P50方面得以恢复活力。P50和2,3 - DPG的恢复取决于磷酸烯醇丙酮酸的浓度。即使经过这些防腐剂处理，红细胞的P50和2,3 - DPG仍会随着储存时间的延长而降低，但储存五周血液的P50和2,3 - DPG仍高于新鲜血液的相应值。用含有磷酸烯醇丙酮酸的防腐剂处理储存血液也会使ATP浓度升高，但储存五周血液中升高的ATP仅约为新鲜血液的50%。通过增加磷酸烯醇丙酮酸浓度无法进一步提高ATP水平，但补充腺嘌呤和核苷可使其得到改善。用15 mM磷酸烯醇丙酮酸孵育储存血液足以使储存三周血液的ATP、2,3 - DPG和P50恢复到接近正常水平。这些研究结果表明，蔗糖对于PEP作为防腐剂添加剂发挥作用并非必需。
