通过将磷酸烯醇丙酮酸掺入细胞中来使衰老红细胞恢复活力。

Rejuvenation of aged erythrocytes by incorporating phosphoenolpyruvate into the cells.

作者信息

Hamasaki N, Minakami S, Ideguchi H, Ikehara Y

出版信息

Acta Biol Med Ger. 1981;40(4-5):691-7.

Abstract

An acid-citrate dextrose solution which contains phosphoenolpyruvate and sucrose was used as a preservative and medium for rejuvenating depleted erythrocytes. 2,3-bisphosphoglycerate and ATP in washed erythrocytes, red cell concentrate or whole blood were increased effectively by incubating the cells with the solution at 37 degrees C for 30--60 min. The transport of phosphoenolpyruvate through the erythrocyte membrane was essential to the increase of 2,3-bisphosphoglycerate and ATP. During storage of the cells at 4 degrees C in the presence of phosphoenolpyruvate, no increase of 2,3-bisphosphoglycerate and ATP was observed because no transport of phosphoenolpyruvate into the cells occurred. By an incubation at 37 degrees C for 30 min at the end of storage periods, however, levels of ATP and 2,3-bisphosphoglycerate in the cells were raised.

摘要

一种含有磷酸烯醇丙酮酸和蔗糖的酸性枸橼酸盐葡萄糖溶液被用作保存剂和使耗尽的红细胞恢复活力的培养基。通过将细胞与该溶液在37℃孵育30 - 60分钟，洗涤过的红细胞、红细胞浓缩液或全血中的2,3 - 二磷酸甘油酸和三磷酸腺苷有效地增加了。磷酸烯醇丙酮酸通过红细胞膜的转运对于2,3 - 二磷酸甘油酸和三磷酸腺苷的增加至关重要。在4℃存在磷酸烯醇丙酮酸的情况下储存细胞时，未观察到2,3 - 二磷酸甘油酸和三磷酸腺苷的增加，因为磷酸烯醇丙酮酸没有转运到细胞中。然而，在储存期结束时通过在37℃孵育30分钟，细胞中的三磷酸腺苷和2,3 - 二磷酸甘油酸水平升高了。