Andersen K B, Nexø B A
Virology. 1983 Feb;125(1):85-98. doi: 10.1016/0042-6822(83)90065-x.
We have studied the entry of murine retrovirus into mouse fibroblasts by following the fate of both radioactively (protein) labeled virus particles and infectious virus particles. Physical and infectious particles bound to the cell surface with a half time of 1.5-2 hr. Both types of particles were internalized with a half time of approximately 3 hr as measured by the resistance to externally added proteases. The binding proceeded both at 37 and 0 degrees, whereas the internalization was blocked at 0 degrees. The internalized physical particles followed two routes: they either were degraded or remained stable in the cell. Degradation was blocked by lysosomotropic bases and is therefore believed to occur in the lysosomes. Infection could also be inhibited by lysosomotropic bases when present in the first hours after the internalization, indicating that the infectious route also is leading through the lysosomes or another acidic compartment of the cell.
我们通过追踪放射性（蛋白质）标记的病毒颗粒和感染性病毒颗粒的命运，研究了鼠逆转录病毒进入小鼠成纤维细胞的过程。物理性和感染性颗粒与细胞表面结合的半衰期为1.5至2小时。通过对外源添加蛋白酶的抗性测定，两种类型的颗粒内化的半衰期约为3小时。结合在37℃和0℃下均会发生，而内化在0℃下被阻断。内化的物理颗粒遵循两条途径：它们要么在细胞内被降解，要么保持稳定。降解被溶酶体促渗碱阻断，因此被认为发生在溶酶体中。当在内化后的最初几小时存在时，溶酶体促渗碱也可以抑制感染，这表明感染途径也通过溶酶体或细胞的另一个酸性区室。
