Barsacchi R, Camici P, Bottigli U, Salvadori P A, Pelosi G, Maiorino M, Ursini F
Biochim Biophys Acta. 1983 Apr 5;762(2):241-7. doi: 10.1016/0167-4889(83)90077-0.
The isolated perfused rat heart emits a spontaneous ultraweak chemiluminescence. When the perfusion is stopped, light emission decreases, indicating the dependency of this phenomenon on aerobic metabolism. Emitted chemiluminescence was markedly enhanced following perfusion with 0.05 mM H2O2 or cumene hydroperoxide or tert-butyl hydroperoxide; substitution of O2 for N2 in the gassing mixture of the perfusion media significantly lowered photon emission. Lipid peroxidation, which is known to be associated with chemiluminescence, was evaluated by HPLC analysis of peroxidized and unperoxidized heart phosphatidylcholines. During hydroperoxide perfusion, coronary flow and heart rate progressively decreased, while lactic dehydrogenase was released after complete cardiac arrest. The resultant morphology of this damage corresponds to the so-called 'stone heart', a pattern already described in both human and experimental pathology.
离体灌注的大鼠心脏会发出自发的超微弱化学发光。当灌注停止时，发光强度降低，这表明该现象依赖于有氧代谢。在用0.05 mM过氧化氢、异丙苯过氧化氢或叔丁基过氧化氢灌注后，发出的化学发光显著增强；在灌注介质的通气混合物中用氮气替代氧气可显著降低光子发射。通过对过氧化和未过氧化的心脏磷脂酰胆碱进行HPLC分析来评估已知与化学发光相关的脂质过氧化。在过氧化氢灌注期间，冠状动脉血流量和心率逐渐降低，而乳酸脱氢酶在心脏完全停搏后释放。这种损伤所导致的形态与所谓的“石心”相对应，这是一种在人类和实验病理学中均已描述过的模式。
