Cadenas E, Arad I D, Fisher A B, Boveris A, Chance B
Biochem J. 1980 Oct 15;192(1):303-9. doi: 10.1042/bj1920303.
Light-emission of the perfused lung is induced by t-butyl hydroperoxide, giving chemiluminescence yields that oscillate between 800 and 1500 counts/s depending on the site and position of the lung. The response of the perfused lung to infusion with different hydroperoxides gives a pattern similar to that observed with the liver microsomal fraction; ethyl hydroperoxide shows a much higher chemiluminescence yield than the tertiary (t-butyl and cumene)hydroperoxides. Alveolar oedema affected the light-emission of the perfused lung depending on the time at which oedema developed, decreasing light emission on infusion of hydroperoxide in the oedematous lung and increasing it when oedema appeared after the maximal chemiluminescence yield was already achieved. Paraquat, administered in vivo, augmented light-emission by approximately 2-fold. The effect of paraquat was a time-dependent process. Lung chemiluminescence, compared with liver chemiluminescence, needed higher hydroperoxide concentration to induce light-emission.
叔丁基过氧化氢可诱导灌注肺发光，根据肺的部位和位置不同，化学发光产率在800至1500计数/秒之间波动。灌注肺对不同氢过氧化物灌注的反应模式与肝微粒体部分观察到的相似；乙基过氧化氢的化学发光产率远高于叔（叔丁基和异丙苯）氢过氧化物。肺泡水肿对灌注肺发光的影响取决于水肿出现的时间，在水肿肺中灌注氢过氧化物时发光减少，而在最大化学发光产率已经达到后出现水肿时发光增加。体内给予百草枯可使发光增强约2倍。百草枯的作用是一个时间依赖性过程。与肝化学发光相比，肺化学发光需要更高浓度的氢过氧化物来诱导发光。
