Clark M R, Mohandas N, Shohet S B
Blood. 1983 May;61(5):899-910.
Whole cell deformability of red cells was measured as a continuous function of suspending medium osmolality using the ektacytometer, a laser-diffraction viscometer. Study of normal cells in which water content and membrane surface area had been selectively modified showed that this technique can detect changes in these properties with high sensitivity. The osmotic deformability profiles obtained from this assay provide information about cell water content, surface area, and the heterogeneity in these cellular properties, information that by conventional methods would require several different types of measurements. Application of this approach to a variety of pathologic blood samples showed that various hematologic disorders can be characterized by the shape of this profile and the position of specific features of the profile along the osmolality axis. Measurement of osmotic deformability profiles thus provides a convenient and comprehensive means of identifying abnormalities either in red cell water content or surface area.
使用激光衍射粘度计——红细胞变形性测定仪，将红细胞的全细胞变形性作为悬浮介质渗透压的连续函数进行测量。对水含量和膜表面积已被选择性改变的正常细胞的研究表明，该技术能够高灵敏度地检测这些特性的变化。通过该测定获得的渗透变形性曲线提供了有关细胞水含量、表面积以及这些细胞特性异质性的信息，而这些信息用传统方法需要进行几种不同类型的测量。将这种方法应用于各种病理性血液样本表明，各种血液学疾病可以通过该曲线的形状以及曲线特定特征沿渗透压轴的位置来表征。因此，测量渗透变形性曲线提供了一种方便且全面的方法来识别红细胞水含量或表面积的异常情况。
