Giavazzi R, Scholar E, Hart I R
Cancer Res. 1983 May;43(5):2216-22.
A variant cell line (UV-2237-ADMR) resistant to the anthracycline antibiotic Adriamycin (Doxorubicin) was selected in vitro from the murine UV-2237 fibrosarcoma tumor cell line. Resistance to Adriamycin proved to be a stable characteristic of the UV-2237-ADMR line, whether the line was grown in vivo or in vitro. The UV-2237-ADMR line also exhibited increased resistance to N-trifluoroacetyladriamycin-24-valerate, daunorubicin, actinomycin D, amsacrine, mitomycin C, vinblastine, and vincristine but not to bleomycin. Cell-cell hybridization studies showed that the Adriamycin resistance is an incompletely dominant trait. Uptake and efflux studies with [14C]Adriamycin indicated that the resistance exhibited by the UV-2237-ADMR line was due to both reduced uptake of the drug and an increased active efflux.
一种对蒽环类抗生素阿霉素（多柔比星）具有抗性的变异细胞系（UV - 2237 - ADMR）是从小鼠UV - 2237纤维肉瘤肿瘤细胞系中体外筛选出来的。对阿霉素的抗性被证明是UV - 2237 - ADMR细胞系的一个稳定特性，无论该细胞系是在体内还是体外培养。UV - 2237 - ADMR细胞系对N - 三氟乙酰阿霉素 - 24 - 戊酸酯、柔红霉素、放线菌素D、安吖啶、丝裂霉素C、长春碱和长春新碱也表现出增强的抗性，但对博来霉素不具有抗性。细胞 - 细胞杂交研究表明，阿霉素抗性是一种不完全显性性状。用[14C]阿霉素进行的摄取和外排研究表明，UV - 2237 - ADMR细胞系表现出的抗性是由于药物摄取减少和主动外排增加所致。
