Hirokawa N, Cheney R E, Willard M
Cell. 1983 Mar;32(3):953-65. doi: 10.1016/0092-8674(83)90080-6.
We have determined that a protein of the fodrin-spectrin-TW260/240 (FST) family is a component of the thin fibrils (approximately 5 nm wide, 100-200 nm long) that cross-link bundles of actin filaments to adjacent actin bundles and to the plasma membrane in the terminal web of the brush border of the intestinal epithelium. When isolated brush borders were incubated with anti-fodrin antibodies and prepared for electron microscopy by the quick-freeze, deep-etch technique, these approximately 5 nm fibrils were specifically decorated with the antibody. In addition, these cross-linking fibrils disappeared when the anti-fodrin-reactive proteins were extracted from the brush border. We conclude that FST is a component of a cross-linking system composed of approximately 5 nm fibrils that are morphologically distinct from the approximately 8 nm myosin-containing fibrils which were identified by anti-myosin decoration. In addition to linking actin bundles to adjacent actin bundles and to the plasma membrane, these FST fibrils may mediate actin-vesicle, actin-intermediate filament and vesicle-plasma membrane linkages.
我们已经确定，血影蛋白-肌动蛋白-TW260/240（FST）家族的一种蛋白质是细纤维（宽约5nm，长100-200nm）的一个组成部分，这些细纤维将肌动蛋白丝束与相邻的肌动蛋白束交联，并与肠上皮刷状缘终末网中的质膜交联。当将分离的刷状缘与抗血影蛋白抗体一起孵育，并通过快速冷冻、深度蚀刻技术制备用于电子显微镜观察时，这些约5nm的细纤维会被该抗体特异性标记。此外，当从刷状缘中提取抗血影蛋白反应性蛋白时，这些交联细纤维会消失。我们得出结论，FST是由约5nm细纤维组成的交联系统的一个组成部分，这些细纤维在形态上与通过抗肌球蛋白标记鉴定出的约8nm含肌球蛋白的细纤维不同。除了将肌动蛋白束与相邻的肌动蛋白束以及质膜连接起来外，这些FST细纤维还可能介导肌动蛋白-囊泡、肌动蛋白-中间丝和囊泡-质膜的连接。
