Gemant A
Mol Biol Rep. 1978 Jun 16;4(2):121-5. doi: 10.1007/BF00775973.
The particles of an iron hydroxide sol were found to be a suitable model for protein-oxidizing enzymes such as peroxidase and polyphenol oxidase. In addition to small molecules such as pyrogallol, human serum proteins, albumin and gamma-globulin, are shown to be substrates of the oxidizing model. The activity is markedly increased by the addition of small amounts of copper to the iron in the particles of the sol. The size and molecular weight of the enzyme model, as well as the number of active centers were determined.
人们发现氢氧化铁溶胶颗粒是过氧化物酶和多酚氧化酶等蛋白质氧化酶的合适模型。除了连苯三酚等小分子外，人血清蛋白、白蛋白和γ-球蛋白也被证明是这种氧化模型的底物。向溶胶颗粒中的铁添加少量铜会显著提高其活性。测定了酶模型的大小、分子量以及活性中心的数量。
