Gilmore M J
Cryobiology. 1983 Feb;20(1):106-10. doi: 10.1016/0011-2240(83)90065-2.
Assays of granulocyte-macrophage colony-forming cells provide a means of testing the viability of cryopreserved bone marrow cells intended for autologous transplantation. We have compared two different sources of granulocyte-macrophage colony-stimulating activity, giant cell tumor-conditioned medium (GCT-CM) and peripheral blood leukocyte feeder layers, to determine whether the former is a suitable substitute for leukocyte feeder layers in the assay. The results show that GCT-CM, while providing a comparable stimulus to that provided by leukocyte feeder layers for colony formation by fresh bone marrow samples, is an inadequate stimulus when cryopreserved bone marrow samples are cultured. GCT-CM is not therefore suitable for use in monitoring cryopreserved bone marrow, since there is gross underestimation of the number of colony-forming cells present when this stimulus is used. The results suggest that great care should be taken when selecting alternative sources of granulocyte-macrophage colony-stimulating activity for culture of cryopreserved material.
粒细胞-巨噬细胞集落形成细胞检测为测试用于自体移植的冷冻保存骨髓细胞的活力提供了一种方法。我们比较了粒细胞-巨噬细胞集落刺激活性的两种不同来源，即巨细胞瘤条件培养基（GCT-CM）和外周血白细胞饲养层，以确定前者在该检测中是否是白细胞饲养层的合适替代品。结果表明，GCT-CM虽然对新鲜骨髓样本的集落形成提供了与白细胞饲养层相当的刺激，但在培养冷冻保存的骨髓样本时，其刺激作用不足。因此，GCT-CM不适用于监测冷冻保存的骨髓，因为使用这种刺激时，集落形成细胞的数量会被严重低估。结果表明，在选择用于培养冷冻保存材料的粒细胞-巨噬细胞集落刺激活性的替代来源时应格外小心。
