Childs G V
Endocrinology. 1983 May;112(5):1647-52. doi: 10.1210/endo-112-5-1647.
The neonatal development of the rat thyrotrope was studied in sections of male rat pituitaries with stereometric techniques on cells that had been identified cytochemically with antirat TSH beta and either the peroxidase-antiperoxidase technique or the avidin-biotin-peroxidase complex technique. The TSH cells were poorly granulated at 2 days of age, and the hormone was stored in pleomorphic saccules or granules. In adult rats, thyrotropes were 4-6% of the pituitary cell population. In the neonatal rat pituitary, the thyrotropes proliferated in clusters, and the percentage of stained cells increased to 18.8% by 9 days of age. The TSH cell percentages then decreased, and the clusters dispersed. The 15-day-old rat pituitaries contained 5% thyrotropes. The TSH cells matured morphologically during the first 10 days of development, and by 7 days, they contained the small granules that are typical of the thyrotrope. After that time, the thyrotropes enlarged and partially degranulated, and TSH beta was stored in some of the large pleomorphic saccules in the cytoplasm. Our studies show that the greater sensitivity to TRH of the thyrotropes in the 5- to 10-day-old rat may be due to the relatively high numbers of thyrotropes in the gland. We suggest that the 9- to 10-day-old male rat pituitary might be useful for a study of separated purified thyrotropes because of its relatively high content of TSH cells.
采用立体测量技术，在雄性大鼠垂体切片上，利用抗大鼠促甲状腺激素β抗体，结合过氧化物酶-抗过氧化物酶技术或抗生物素蛋白-生物素-过氧化物酶复合物技术，对大鼠促甲状腺细胞进行细胞化学鉴定，研究其新生期发育情况。促甲状腺细胞在2日龄时颗粒较少，激素储存在多形性囊泡或颗粒中。成年大鼠中，促甲状腺细胞占垂体细胞总数的4%-6%。在新生大鼠垂体中，促甲状腺细胞成簇增殖，到9日龄时，染色细胞百分比增至18.8%。之后促甲状腺细胞百分比下降，细胞簇分散。15日龄大鼠垂体中促甲状腺细胞占5%。促甲状腺细胞在发育的前10天内形态成熟，到7日龄时，它们含有促甲状腺细胞特有的小颗粒。此后，促甲状腺细胞增大并部分脱颗粒，促甲状腺激素β储存在细胞质中的一些大型多形性囊泡中。我们的研究表明，5至10日龄大鼠的促甲状腺细胞对促甲状腺激素释放激素的敏感性较高，可能是由于腺体内促甲状腺细胞数量相对较多。我们认为，9至10日龄雄性大鼠垂体因其促甲状腺细胞含量相对较高，可能有助于分离纯化促甲状腺细胞的研究。
