Dacheux F
Cell Tissue Res. 1984;235(3):623-33. doi: 10.1007/BF00226961.
The fetal porcine pituitary was investigated by means of ultrastructural immunocytochemistry (1) to identify the first cells synthesizing the adenohypophyseal hormones, (2) to follow their differentiation during fetal development, and (3) to compare their ultrastructural characteristics with those of mature adult cells. The first ACTH-cells, which produced and stored ACTH, beta-LPH, beta-MSH, and alpha- and beta-endorphin in the same granules, were very numerous at day 34 and displayed a uniform morphology. At day 50 and thereafter, until the end of gestation, the ACTH-cells differed in their appearance probably reflecting various stages of differentiation of one cell type. The GH-cells gained rapidly ultrastructural features comparable to those of mature GH-cells. In contrast, in the case of PRL-cells, which appeared only at the end of the gestation period as immature elements containing very small secretory granules, the morphological maturation seemed to take place only after birth. The first cells synthesizing the glycoprotein hormones (LH alpha, LH beta, FSH and TSH) displayed ultrastructural features of immature cells. At day 50, their ultrastructural organization started to show a different pattern. At the end of gestation, the TSH-cells and the gonadotropic cells displayed the ultrastructural features of mature cells.
利用超微结构免疫细胞化学方法对胎猪垂体进行了研究，目的如下：（1）鉴定最早合成腺垂体激素的细胞；（2）追踪这些细胞在胎儿发育过程中的分化情况；（3）将它们的超微结构特征与成年成熟细胞的特征进行比较。最早的促肾上腺皮质激素（ACTH）细胞能在同一颗粒中产生并储存ACTH、β-促脂素（β-LPH）、β-促黑素（β-MSH）以及α-和β-内啡肽，在第34天时数量众多，形态一致。在第50天及之后，直至妊娠末期，ACTH细胞的外观有所不同，这可能反映了一种细胞类型分化的不同阶段。生长激素（GH）细胞迅速获得了与成熟GH细胞相当的超微结构特征。相比之下，催乳素（PRL）细胞直到妊娠期结束时才作为含有非常小的分泌颗粒的未成熟细胞出现，其形态成熟似乎仅在出生后才发生。最早合成糖蛋白激素（促黄体生成素α亚基、促黄体生成素β亚基、促卵泡生成素和促甲状腺激素）的细胞表现出未成熟细胞的超微结构特征。在第50天时，它们的超微结构组织开始呈现出不同的模式。在妊娠末期，促甲状腺激素细胞和促性腺激素细胞表现出成熟细胞的超微结构特征。
