Domdey H, Wiebauer K, Klapthor H, Arnold H H
Eur J Biochem. 1983 Mar 1;131(1):129-35. doi: 10.1111/j.1432-1033.1983.tb07239.x.
Using a cloned cDNA (pGAP30) the nucleotide sequence for chicken glyceraldehyde-3-phosphate dehydrogenase mRNA has been determined. The cDNA insert contains 1051 nucleotides representing the amino acid coding sequence, with the exception of 49 NH2-terminal amino acids, and includes the entire 3'-noncoding region. Sequence information on the missing 5' terminus of the mRNA, not represented in the clone pGAP30, was obtained by extension of the cDNA using an 85-nucleotide-long internal fragment as a primer. Thus the sequence of 310 amino acids of chicken glyceraldehyde-3-phosphate dehydrogenase representing 93% of the complete primary structure could be derived. The coding portion exhibits non-random utilization of synonymous codons with a strong bias for codons with G or C at the third position. The non-coding region contains several octanucleotides which are repeated and shows a potentially stable stem-and-loop structure located towards the end of the mRNA. Hypothetical functional implications of the putative secondary structure are discussed.
利用克隆的互补DNA（pGAP30），已确定了鸡甘油醛-3-磷酸脱氢酶信使核糖核酸（mRNA）的核苷酸序列。该cDNA插入片段包含1051个核苷酸，代表氨基酸编码序列，但不包括49个氨基末端氨基酸，并且包含整个3'-非编码区。通过使用一个85个核苷酸长的内部片段作为引物延伸cDNA，获得了克隆pGAP30中未代表的mRNA缺失5'末端的序列信息。因此，可以推导鸡甘油醛-3-磷酸脱氢酶310个氨基酸的序列，占完整一级结构的93%。编码部分对同义密码子的使用并非随机，对第三位带有G或C的密码子有强烈偏好。非编码区包含几个重复的八核苷酸，并在mRNA末端附近显示出一个潜在稳定的茎环结构。讨论了假定二级结构的假设功能意义。
