Arnold H H, Krauskopf M, Siddiqui M A
Nucleic Acids Res. 1983 Feb 25;11(4):1123-31. doi: 10.1093/nar/11.4.1123.
The nucleotide sequence of a cDNA clone (pML10) for chicken cardiac myosin light chain is described. The cDNA insert contains 613 nucleotides representing the entire coding sequence, with the exception of nine NH2-terminal amino acids, and the full 3'-non-coding region of 146 nucleotides. The missing 5' terminus of the mRNA, not represented in the clone pML10, was obtained by extension of the cDNA using a 43 nucleotide long internal EcoR1 fragment as a primer. The non-coding region contains several direct and inverted repeated sequences and the polyadenylation signal sequence AATAAA. The coding portion exhibits non-random usage of synonymous codons with a strong bias for codons ending in G and C.
本文描述了鸡心肌肌球蛋白轻链的一个cDNA克隆（pML10）的核苷酸序列。该cDNA插入片段包含613个核苷酸，代表了整个编码序列，但不包括九个NH2末端氨基酸，以及146个核苷酸的完整3'-非编码区。通过使用一个43个核苷酸长的内部EcoR1片段作为引物来延伸cDNA，获得了克隆pML10中未代表的mRNA缺失的5'末端。非编码区包含几个正向和反向重复序列以及聚腺苷酸化信号序列AATAAA。编码部分表现出同义密码子的非随机使用，对以G和C结尾的密码子有强烈偏好。
