Rew R S, Douvres F W, Saz H J
Exp Parasitol. 1983 Apr;55(2):179-87. doi: 10.1016/0014-4894(83)90012-7.
Larval stages of Oesophagostomum radiatum grown in vitro and adults grown in vivo were incubated in complex media or in a simple salt solution containing radioactive glucose. Glucose disappearance and end product accumulation of third-stage larvae in a simple salt solution indicated that they excreted CO2 and acetic, propionic, and lactic acids. Larvae in third molt, fourth stage, and adults all excreted CO2, acetic, propionic, and lactic acids at twice the rate of third-stage larvae plus an additional product, methylbutyric acid. Carbon dioxide arose primarily from the 3 or 4 carbons of glucose. An anaerobic atmosphere (95% N2:5% CO2) had no apparent effect on metabolism. When incubation was done in complex media, isobutyric and 3-methylbutyric acids were seen as major excretion products (10 and 24%, respectively). However, these acids were quantitatively minor when incubations took place in simple salts-glucose medium (1 and 0-3%, respectively).
将体外培养的辐射食道口线虫幼虫和体内生长的成虫置于复合培养基或含有放射性葡萄糖的简单盐溶液中进行孵育。在简单盐溶液中，第三期幼虫的葡萄糖消失和终产物积累表明它们排泄出二氧化碳以及乙酸、丙酸和乳酸。处于第三次蜕皮期、第四期的幼虫以及成虫均排泄出二氧化碳、乙酸、丙酸和乳酸，排泄速率是第三期幼虫的两倍，并且还排泄出另一种产物甲基丁酸。二氧化碳主要源自葡萄糖的3个或4个碳原子。厌氧环境（95%氮气：5%二氧化碳）对新陈代谢无明显影响。在复合培养基中进行孵育时，异丁酸和3-甲基丁酸是主要的排泄产物（分别占10%和24%）。然而，在简单盐-葡萄糖培养基中进行孵育时，这些酸在数量上较少（分别占1%和0-3%）。
