从小鼠卵巢卵母细胞中大规模分离透明带。
Large scale isolation of zonae pellucidae from ovarian oocytes of mice.
作者信息
Brauer M M, Sotelo J R
出版信息
Experientia. 1983 Apr 15;39(4):420-1. doi: 10.1007/BF01963159.
PMID:6832328
Abstract
A large scale method for the isolation of zonae pellucidae (ZP) from mouse ovaries is described. It involves the squashing of the ovaries on a screen (70 X 70 M mesh) and sedimentation of the material in a discontinuous gradient. It is possible to obtain free ZP in useful quantities in about 1 h's work.
摘要
本文描述了一种从小鼠卵巢中分离透明带（ZP）的大规模方法。该方法包括将卵巢在筛网（70×70微米目）上挤压，以及使材料在不连续梯度中沉降。通过约1小时的工作，有可能获得大量可用的游离透明带。
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