Thomas W E, Ellar D J
FEBS Lett. 1983 Apr 18;154(2):362-8. doi: 10.1016/0014-5793(83)80183-5.
Bacillus thuringiensis var israelensis delta-endotoxin protein active against mosquitoes was inactivated by prior incubation with lipids extracted from Aedes albopictus cells. Experiments with lipid dispersions and multilamellar liposomes showed that the toxin binds to phosphatidyl choline, sphingomyelin and phosphatidyl ethanolamine provided these lipids contain unsaturated fatty acids. Phosphatidyl serine binds toxin less efficiently and phosphatidyl inositol, cardiolipin, cerebroside and cholesterol show no affinity for the toxin. The results suggest an insecticidal mechanism in which interaction of toxin with affinity for the toxin. The results suggest an insecticidal mechanism in which interaction of toxin with specific plasma membrane lipids causes a detergent-like rearrangement of the lipids, leading to disruption of membrane integrity and eventual cytolysis.
对蚊子有活性的苏云金芽孢杆菌以色列变种δ-内毒素蛋白，在与从白纹伊蚊细胞中提取的脂质预先温育后会失活。脂质分散体和多层脂质体实验表明，该毒素能与磷脂酰胆碱、鞘磷脂和磷脂酰乙醇胺结合，前提是这些脂质含有不饱和脂肪酸。磷脂酰丝氨酸与毒素的结合效率较低，而磷脂酰肌醇、心磷脂、脑苷脂和胆固醇对该毒素无亲和力。结果提示了一种杀虫机制，即毒素与对其有亲和力的特定质膜脂质相互作用，导致脂质发生类似去污剂的重排，从而破坏膜的完整性并最终导致细胞溶解。
