Goldsmith M A, Huling S, Jones A L
Gastroenterology. 1983 May;84(5 Pt 1):978-86.
17 alpha-Ethynyl estradiol-induced cholestasis was used to study the relationship of protein to bile salt transport in liver. The biliary secretion of horseradish peroxidase was unaltered in treated animals despite a 56% reduction in bile flow. Cytochemistry confirmed that estradiol caused no alteration in the handling of tracer. In a second study, the peak biliary secretion of [14C]taurocholate was reduced by approximately 46% in treated animals. The kinetics of 125I-cholyglycylhistamine, a bile salt derivative, were identical to those of taurocholate in control and cholestatic animals. Taurocholate and cholylglycylhistamine secretion were markedly reduced in control animals during competition with unlabeled taurocholate. Quantitative electron microscopic autoradiography with 125I-cholylglycylhistamine revealed a high concentration of grains over the endoplasmic reticulum and the Golgi complex including associated lysosomes and vesicles. These data demonstrate that estradiol markedly inhibits bile salt transport, but not vesicular transport of horseradish peroxidase. Furthermore, estradiol may alter the movement of bile salts through these organelles.
17α-乙炔雌二醇诱导的胆汁淤积用于研究肝脏中蛋白质与胆盐转运的关系。尽管胆汁流量减少了56%，但经处理动物中辣根过氧化物酶的胆汁分泌未发生改变。细胞化学证实雌二醇对示踪剂的处理没有影响。在第二项研究中，经处理动物中[14C]牛磺胆酸盐的胆汁分泌峰值降低了约46%。胆盐衍生物125I-胆酰甘氨酰组胺在对照动物和胆汁淤积动物中的动力学与牛磺胆酸盐相同。在与未标记的牛磺胆酸盐竞争时，对照动物中牛磺胆酸盐和胆酰甘氨酰组胺的分泌明显减少。用125I-胆酰甘氨酰组胺进行的定量电子显微镜放射自显影显示，在内质网和高尔基体包括相关的溶酶体和小泡上有高浓度的银粒。这些数据表明，雌二醇显著抑制胆盐转运，但不抑制辣根过氧化物酶的囊泡转运。此外，雌二醇可能会改变胆盐通过这些细胞器的移动。
