Rahman K, Coleman R
Department of Biochemistry, University of Birmingham, U.K.
Biochem J. 1987 Jul 15;245(2):531-6. doi: 10.1042/bj2450531.
Multiple short pulses of taurocholate (TC) brought about, in isolated perfused rat livers, the secretion of phospholipid and cholesterol into bile; the lipids showed an appreciable lag period behind the bile-salt secretion, and there was considerable variability in response, both between low and high dose pulses of TC and, at the higher dose, even between individual livers. When a background continuous infusion of taurodehydrocholate (a hydrophilic non-micelle-forming bile-salt analogue) was superimposed upon the short TC pulses, the lipid secretion showed much better control, and the lipid peaks were of more uniform size, following more closely, or more coincident with, the bile-salt output peaks. Taurodehydrocholate may provide a signal for the control of the supply and delivery of lipid vesicles to the bile-canalicular membrane, from where the lipid vesicles are then removed by the action of the pulses of TC.
在离体灌注大鼠肝脏中，多次短脉冲牛磺胆酸盐（TC）可促使磷脂和胆固醇分泌到胆汁中；这些脂质在胆盐分泌后有明显的延迟期，并且无论是在低剂量和高剂量TC脉冲之间，还是在高剂量时，即使在各个肝脏之间，反应都存在相当大的变异性。当在短TC脉冲基础上叠加牛磺去氢胆酸盐（一种亲水性非形成微胶粒的胆盐类似物）的背景持续输注时，脂质分泌显示出更好的控制，脂质峰的大小更均匀，更紧密地跟随或更与胆盐输出峰重合。牛磺去氢胆酸盐可能为脂质囊泡向胆小管膜的供应和输送控制提供信号，随后脂质囊泡通过TC脉冲的作用从胆小管膜被清除。
