Baruch A L, Yedwab G, David M P, Homonnai Z T, Paz G F
Gynecol Obstet Invest. 1983;15(3):163-70. doi: 10.1159/000299407.
Midcycle cervical mucus was obtained from 56 women. Each mucus sample was divided into four portions collected into a capillary tube and kept either at room temperature or at 4 degrees C, -20 degrees C or in liquid nitrogen for 1 h, afterwards thawed at 37 degrees C and used in the in vitro penetration device for 1 h. 56 semen samples of good quality were used, the same sample for all four types of the same cervical mucus. Very high significant correlations were calculated between the penetration value (PV) rates in all four methods of cervical mucus preservation. Paired t test revealed a significant decrease (p less than 0.025) in the PV rates of sperm in the cervical mucus cryopreserved in liquid nitrogen. The results of this study recommend the establishment of a cervical mucus bank using the 4 degrees C and -20 degrees C as possible methods for preservation of cervical mucus.
从56名女性中获取了月经周期中期的宫颈黏液。每个黏液样本被分成四份，收集到毛细管中，分别保存在室温、4℃、-20℃或液氮中1小时，之后在37℃解冻，并在体外渗透装置中使用1小时。使用了56份质量良好的精液样本，对同一宫颈黏液的所有四种类型使用相同的样本。计算了宫颈黏液所有四种保存方法的渗透值（PV）率之间的非常高度显著的相关性。配对t检验显示，液氮冷冻保存的宫颈黏液中精子的PV率显著降低（p<0.025）。本研究结果建议建立一个宫颈黏液库，使用4℃和-20℃作为保存宫颈黏液的可能方法。
