Eckels K H, Summers P L, Russell P K
Infect Immun. 1983 Feb;39(2):750-4. doi: 10.1128/iai.39.2.750-754.1983.
Temperature-sensitive events occurring during the replication of the attenuated S-1 clone of dengue type 2 virus were examined. The S-1 clone was more thermolabile than the parent virus at the nonpermissive temperature of 38.5 degrees C. Adsorption experiments in fetal rhesus monkey lung cells revealed an inefficient adsorption of S-1 at 38.5 degrees C compared with the parent virus, suggesting an alteration in a thermolabile virion protein important in adsorption. The production of S-1 viral RNA and antigen occurred at the nonpermissive temperature, which indicated that early events in the replication cycle of S-1 were not affected. Release of infectious virus at 38.5 degrees C was not impaired; however, lower amounts of infectious virus in infected cells at the nonpermissive temperature indicated that maturation of the S-1 clone was suppressed.
对登革2型病毒减毒S-1克隆株复制过程中发生的温度敏感事件进行了研究。在38.5℃的非允许温度下，S-1克隆株比亲代病毒更不耐热。在恒河猴胎儿肺细胞中进行的吸附实验表明，与亲代病毒相比，S-1在38.5℃时吸附效率低下，这表明在吸附过程中起重要作用的一种不耐热病毒体蛋白发生了改变。S-1病毒RNA和抗原的产生在非允许温度下发生，这表明S-1复制周期的早期事件未受影响。在38.5℃时传染性病毒的释放未受损；然而，在非允许温度下感染细胞中传染性病毒数量较少，表明S-1克隆株的成熟受到抑制。
