Tanaka T, Rubin S, Sun S T, Nishio I, Tung W H, Chylack L T
Invest Ophthalmol Vis Sci. 1983 Apr;24(4):522-5.
Experimental evidence that indicates the nuclear opacity that develops in the hypoglycemic cataract in rat lenses is the result of a phase separation of the lens cytoplasm is presented. Phase diagrams of the cytoplasm of organ-cultured lenses were constructed for a series of incubation times in normal and hypoglycemic media. In normal media, the maximum phase separation temperature, Tmax, of the lens cytoplasm decreases with time. In hypoglycemic media, Tmax increases with time and exceeds 37 C after 24 hrs. The increase in Tmax can be stopped and reversed if the lens is returned to normal media within 8 hrs; in good agreement with an earlier biochemical analysis of the kinetics of hypoglycemic cataract formation.
本文提供了实验证据，表明大鼠晶状体低血糖性白内障中出现的核不透明是晶状体细胞质相分离的结果。针对在正常和低血糖培养基中一系列培养时间构建了器官培养晶状体细胞质的相图。在正常培养基中，晶状体细胞质的最大相分离温度Tmax随时间降低。在低血糖培养基中，Tmax随时间升高，并在24小时后超过37℃。如果晶状体在8小时内恢复到正常培养基中，Tmax的升高可以停止并逆转；这与早期对低血糖性白内障形成动力学的生化分析结果高度一致。
