Ryan A F, Schwartz I R
Hear Res. 1983 Feb;9(2):173-84. doi: 10.1016/0378-5955(83)90026-6.
The localization of 3H-labeled amino acids was compared by light and electron microscopic autoradiography in the spiral ganglion of the gerbil, following in vivo intracochlear incubations. Following incubations with taurine, a population of heavily labeled neurons could be distinguished from lightly labeled neurons. The heavily labeled population comprised 5-6% of spiral ganglion neurons, and included the least myelinated cells. Ultrastructurally, the heavily labeled neurons were characterized by a loosely coiled Schwann cell sheath covering the cell body, and the presence of abundant cytoplasmic microfilaments. The unlabeled cells showed a typical perikaryal myelin sheath and cytoplasm rich in rough endoplasmic reticulum. It is concluded that the spiral ganglion of the gerbil contains both Type I and Type II neurons, and that Type II neurons preferentially incorporate the amino acid taurine. Type II neurons are therefore biochemically as well as morphologically distinct from Type I neurons.
在体内进行耳蜗内孵育后，通过光学显微镜和电子显微镜放射自显影术比较了沙鼠螺旋神经节中3H标记氨基酸的定位。在用牛磺酸孵育后，可以区分出一群标记较重的神经元和标记较轻的神经元。标记较重的群体占螺旋神经节神经元的5-6%，包括髓鞘最少的细胞。超微结构上，标记较重的神经元的特征是细胞体被松散盘绕的施万细胞鞘覆盖，并且存在丰富的细胞质微丝。未标记的细胞显示出典型的核周髓鞘和富含粗面内质网的细胞质。得出的结论是，沙鼠的螺旋神经节包含I型和II型神经元，并且II型神经元优先摄取氨基酸牛磺酸。因此，II型神经元在生化和形态上均与I型神经元不同。
