Farach M C, Martinez-Carrion M
J Biol Chem. 1983 Apr 10;258(7):4166-70.
Various acetylcholine receptor-rich membrane preparations from Torpedo californica electroplax tissue were examined using the techniques of differential scanning calorimetry coupled with gel electrophoretic analysis of heat-denaturing material and functional assays following passage through discrete transitions. In unfractionated membranes, four irreversible calorimetric transitions were observed, one of which (Td = 59 degrees C) could be assigned to a complete loss of acetylcholine receptor function. A second lower temperature transition apparently corresponds to loss of certain peripheral membrane proteins including the Mr = 43,000 polypeptide and the acetylcholinesterase activity. Membrane preparations highly enriched in acetylcholine receptor polypeptides contained a major transition at 59 degrees C which could be shown to be sensitive to the presence of added ligands of the acetylcholine receptor, supporting its assignment to structural alterations of the receptor protein or its arrangement in the membrane.
利用差示扫描量热法技术，结合对热变性材料的凝胶电泳分析以及经过离散转变后的功能测定，对来自加州电鳐电板组织的各种富含乙酰胆碱受体的膜制剂进行了检测。在未分级的膜中，观察到四个不可逆的量热转变，其中一个转变（Td = 59℃）可归因于乙酰胆碱受体功能的完全丧失。第二个较低温度的转变显然对应于某些外周膜蛋白的丧失，包括分子量为43,000的多肽和乙酰胆碱酯酶活性。高度富集乙酰胆碱受体多肽的膜制剂在59℃有一个主要转变，该转变可被证明对添加的乙酰胆碱受体配体的存在敏感，这支持了将其归因于受体蛋白的结构改变或其在膜中的排列。
