Connolly J A, St John P A, Fischbach G D
J Neurosci. 1982 Sep;2(9):1207-13. doi: 10.1523/JNEUROSCI.02-09-01207.1982.
Saline extracts prepared from the electric lobe, the electromotor nerves, and the electric organ (the electromotor system) of Torpedo californica increase the number of ACh receptors on uninnervated chick myotubes in culture, while extracts from T. californica liver or skeletal muscle do not. The extracts also increase the ACh sensitivity of treated myotubes, indicating that newly synthesized receptors are functional. The active substance(s) is heat sensitive but not trypsin sensitive. Gel filtration on Bio-Gel P-150 shows that the activity is associated with a peak of low (less than 5,000-dalton) molecular weight activity. Labeling studies with rhodamine-conjugated alpha-bungarotoxin show that, in addition to their effect on receptor number, these extracts also cause aggregation of prelabeled ACh receptors on the myotube surface.
从加州电鳐的电叶、电动神经和电器官（电动系统）制备的盐提取物可增加培养中未受神经支配的鸡肌管上乙酰胆碱（ACh）受体的数量，而加州电鳐肝脏或骨骼肌的提取物则无此作用。这些提取物还增加了经处理肌管对ACh的敏感性，表明新合成的受体具有功能。活性物质对热敏感，但对胰蛋白酶不敏感。在Bio-Gel P-150上进行凝胶过滤显示，活性与低分子量（小于5000道尔顿）活性峰相关。用罗丹明偶联的α-银环蛇毒素进行的标记研究表明，除了对受体数量的影响外，这些提取物还会导致预先标记的ACh受体在肌管表面聚集。
