Gillan F T, Johns R B
J Chromatogr Sci. 1983 Jan;21(1):34-8. doi: 10.1093/chromsci/21.1.34.
A facile, reproducible HPLC procedure for the analysis of algal and bacterial carotenoids has been developed. By selection of appropriate detection wavelengths, it is possible to determine wavelength ratios that typify selected carotenoids and permit rapid distinction between coeluting pigments with different numbers of conjugated double bonds. The concurrent isolation of individual neutral lipid classes with the carotenoid analysis is also demonstrated.
已开发出一种简便、可重复的高效液相色谱法（HPLC），用于分析藻类和细菌类胡萝卜素。通过选择合适的检测波长，可以确定代表所选类胡萝卜素的波长比，并能快速区分具有不同共轭双键数的共洗脱色素。同时还展示了在进行类胡萝卜素分析的同时对各个中性脂质类别的分离。
