Shoobridge R, Velkou D, McCredie J
J Exp Zool. 1983 Jan;225(1):73-87. doi: 10.1002/jez.1402250110.
Left caudal neural crest was ablated by diathermy in 2-day-old chick embryos, to injure the region of sensory sciatic innervation before the appearance of the hind-limb bud. Embryos were fixed immediately, 24 hr and 14 days later, for examination of the effects of the ablation upon neural crest, neural tube, somitic mesoderm, and subsequent limb development. All embryos examined immediately and at 24 hr after ablation showed damage to the left neural crest, but the injury extended deeper into neural fold or tube, across the midline, and into somitic mesoderm. Of 17 chicks which survived to 16 days, 7 had gross deformity of the left leg and 10 had morphologically normal legs. Deformed chicks had a significant reduction in the mass of dorsal root ganglia on the operated side but no reduction in the area of the spinal cord. Operated chicks without limb deformities showed reduction of spinal cord but not of dorsal root ganglia. This suggests that limb morphogenesis may be quantitatively related to neural crest but not to neural tube. This possibility is considered in the light of previous publications. However, the experimental limitation of the diathermy method leaves the final conclusion open to interesting speculation and to future examination by improved experimental design.
在2日龄鸡胚中，通过透热法消融左尾侧神经嵴，以在 hind - limb bud出现之前损伤坐骨神经感觉支配区域。分别在消融后立即、24小时和14天对胚胎进行固定，以检查消融对神经嵴、神经管、体节中胚层以及后续肢体发育的影响。所有在消融后立即和24小时检查的胚胎均显示左神经嵴受损，但损伤深入到神经褶或神经管更深部位，穿过中线并累及体节中胚层。在存活至16天的17只小鸡中，7只左腿有明显畸形，10只腿部形态正常。畸形小鸡手术侧背根神经节质量显著减少，但脊髓面积未减少。无肢体畸形的手术小鸡脊髓面积减少，但背根神经节未减少。这表明肢体形态发生可能在数量上与神经嵴有关，而与神经管无关。根据先前的出版物考虑了这种可能性。然而，透热法的实验局限性使得最终结论有待有趣的推测以及通过改进实验设计进行未来研究。
