Nony C R, Althaus J R, Bowman M C
J Anal Toxicol. 1983 Jan-Feb;7(1):40-8. doi: 10.1093/jat/7.1.40.
Specific, precise and sensitive methods are described for determining traces of potentially carcinogenic metabolites of Direct Red 2 and Direct Blue 15 in rat and hamster urine and to monitor the urine from workers who may be occupationally exposed to these dyes. This methodology is generally applicable to metabolites of azo dyes based on benzidine or three of its congeners. The benzidine-congener free amines, their mono and diacetylated analogs and alkaline hydrolyzable conjugates were determined after appropriate extraction and hydrolysis by HPLC or EC/GC. Residues of metabolites in rat, hamster, and human urine were determined at levels as low as 1 ppb. Supplementary information is also presented concerning hydrolysis of diacetylated metabolites and the stability of Direct Red 2 and Direct Blue 15 in rat, hamster and human urine.
本文描述了用于测定大鼠和仓鼠尿液中直接红2和直接蓝15潜在致癌代谢物痕量的特定、精确且灵敏的方法，以及用于监测可能职业接触这些染料的工人尿液的方法。该方法一般适用于基于联苯胺或其三种同系物的偶氮染料的代谢物。在适当萃取和水解后，通过高效液相色谱法（HPLC）或电化学/气相色谱法（EC/GC）测定联苯胺同系物游离胺、其一乙酰化和二乙酰化类似物以及碱性可水解结合物。在大鼠、仓鼠和人类尿液中，代谢物残留的测定下限低至1 ppb。此外，还提供了关于二乙酰化代谢物水解以及直接红2和直接蓝15在大鼠、仓鼠和人类尿液中的稳定性的补充信息。
