Sturdee A P, Beirne J M, Sörman A E, Orton T C, Crisp D M
Life Sci. 1983 Mar 28;32(13):1463-9. doi: 10.1016/0024-3205(83)90912-8.
The metabolic capability of hepatocytes prepared by the perfusion method (P cells) and the tissue slice method (S cells) has been compared using standardised procedures. Yields of P cells were four times greater than for S cells. Trypan blue exclusion viability and oxygen utilization were similar although the viability of S cells deteriorated faster with time. P cells had a lower endogenous rate of glycogenolysis and showed better glucagon stimulation than S cells. Similarly, P cells performed gluconeogenesis at a higher rate. However, there was no significant differences in the metabolism of the xenobiotic ethoxycoumarin. It is concluded that while S cells are probably satisfactory for studies of drug metabolism their use for work involving surface receptor binding and energy demanding processes should be questioned.
采用标准化程序比较了通过灌注法制备的肝细胞（P细胞）和组织切片法制备的肝细胞（S细胞）的代谢能力。P细胞的产量是S细胞的四倍。台盼蓝排斥法检测的活力和氧利用率相似，尽管S细胞的活力随时间下降得更快。P细胞的内源性糖原分解速率较低，并且与S细胞相比，对胰高血糖素刺激的反应更好。同样，P细胞进行糖异生的速率更高。然而，对外源性乙氧香豆素的代谢没有显著差异。得出的结论是，虽然S细胞可能适合用于药物代谢研究，但对于涉及表面受体结合和能量需求过程的研究，其适用性值得质疑。
