年龄对麂属动物姐妹染色单体交换频率及X射线诱导的染色体畸变的影响。

Influence of age on the frequency of sister-chromatid exchanges and X-ray-induced chromosome aberrations in muntjac.

作者信息

Das B C, Sharma T

出版信息

Mutat Res. 1983 Apr;109(1):53-63. doi: 10.1016/0027-5107(83)90094-5.

Abstract

The BrdU-differential staining technique was used in a study of the frequency of sister-chromatid exchanges (SCEs) and X-ray-induced chromosome aberrations in peripheral blood lymphocytes of the same individual muntjacs. Blood was collected periodically from immediately after birth (1 day old) to the adult stage (1 year). The results showed that both the frequency of base-line SCEs and induced chromosome aberrations changed as a function of age. At a young age, the frequency of SCEs was significantly low, whereas a high frequency of chromosome aberrations was observed. But with increase in age of the individuals, an enhanced frequency of SCEs and a decreased frequency of induced chromosome aberrations were observed; and as the age advanced further, the frequencies of both SCEs and chromosomal aberrations came to a steady level.

摘要

采用BrdU差异染色技术研究了同一雄性麂外周血淋巴细胞中姐妹染色单体交换（SCE）频率和X射线诱导的染色体畸变情况。从出生后即刻（1日龄）到成年期（1岁）定期采集血液。结果表明，基线SCE频率和诱导的染色体畸变频率均随年龄变化。在幼年时，SCE频率显著较低，而观察到较高频率的染色体畸变。但随着个体年龄的增加，SCE频率增加，诱导的染色体畸变频率降低；随着年龄进一步增长，SCE和染色体畸变的频率均达到稳定水平。