Crossen P E, Morgan W F
Mutat Res. 1979 Aug;62(1):125-9. doi: 10.1016/0027-5107(79)90225-2.
The incidence of Sister-Chromatid Exchanges (SCEs) due to beta-radiation was investigated in cultured human lymphocytes using the BrdU/Giemsa technique. Cultures treated continuously with 0.001 and 0.01 microCi of [3H]uridine showed no increase in either chromosome abnormalities or SCEs. Continuous treatment with 0.1 microCi resulted in a significant increase in chromosome aberrations but no increase in SCEs, while treatment with 0.2 microCi gave both an increase in chromosome aberrations and SCEs. Cultures given a 4-h pulse with 1.0 microCi showed a significant increase in both SCEs and chromosome aberrations. The results indicate that low levels of beta-radiation do not cause an increase in SCEs in human lymphocytes, and, that a number, if not all the exchanges observed at low levels of beta-radiation with autoradiography, may be spontaneous events.
利用BrdU/吉姆萨技术，在培养的人淋巴细胞中研究了β辐射导致的姐妹染色单体交换（SCEs）发生率。用0.001和0.01微居里的[3H]尿苷连续处理培养物，染色体异常或SCEs均未增加。用0.1微居里连续处理导致染色体畸变显著增加，但SCEs未增加，而用0.2微居里处理则使染色体畸变和SCEs均增加。用1.0微居里进行4小时脉冲处理的培养物中，SCEs和染色体畸变均显著增加。结果表明，低水平的β辐射不会导致人淋巴细胞中SCEs增加，并且，在低水平β辐射下通过放射自显影观察到的一些（如果不是全部）交换可能是自发事件。
