Proffitt R T, Williams L E, Presant C A, Tin G W, Uliana J A, Gamble R C, Baldeschwieler J D
Science. 1983 Apr 29;220(4596):502-5. doi: 10.1126/science.6836294.
The reticuloendothelial system of mice bearing EMT6 tumors was effectively blocked by intravenous injections of small unilamellar vesicles that incorporated a 6-aminomannose derivative of cholesterol in the lipid bilayer. Neutral liposomes loaded with indium-111-nitrilotriacetic acid were then injected. Fifty percent more radioactivity was deposited in tumors of the animals with blocked reticuloendothelial systems than in controls. Twenty-four hours after the injection of radioactive vesicles, well-defined tumor images were observed in whole-body gamma camera scintigraphs. Biodistribution studies showed that tumors from animals with blocked reticuloendothelial systems had more than twice the radioactivity per gram than any other tissue analyzed.
通过静脉注射在脂质双层中掺入胆固醇的6-氨基甘露糖衍生物的小单层囊泡，可有效阻断携带EMT6肿瘤小鼠的网状内皮系统。然后注射负载铟-111-次氮基三乙酸的中性脂质体。网状内皮系统被阻断的动物肿瘤中沉积的放射性比对照组多50%。注射放射性囊泡24小时后，在全身γ相机闪烁扫描图中观察到清晰的肿瘤图像。生物分布研究表明，网状内皮系统被阻断的动物的肿瘤每克放射性是所分析的任何其他组织的两倍多。
