Asato R, Handa H, Hashi T, Hatta J, Komoike M, Yazaki T
Stroke. 1983 Mar-Apr;14(2):191-7. doi: 10.1161/01.str.14.2.191.
Two experiments were done with a prototype mini-NMR imager to evaluate the potential application of nuclear magnetic resonance (NMR) imaging in neuropathology. Cryo-injury-induced brain edema in brain slices from 22 adult male rats was imaged for observing the chronological sequences. Blood-brain barrier permeability changes were evaluated in 12 other brain slice images. EDTA-2Na-Mn solution was intravenously injected as an indicator of blood-brain barrier permeability. Contrast enhancement was achieved by changing the NMR imaging parameters. High resolution imaging permitted visualization of the corpus callosum, the thickness of which was only 0.2-0.4 mm. The extent of edema in gray matter was clearly shown with a striking contrast; no consistent findings were seen with slight differences in water content between edema and the surrounding normal cortex. As a result, the chronological sequences of brain edema were clearly observed. Mn-EDTA leaking from the circulating blood through the damaged capillary wall had a "paradoxical enhancement" effect on the NMR images; this effect might be suitable for evaluating blood-brain barrier permeability changes in NMR images.
使用一台原型微型核磁共振成像仪进行了两项实验，以评估核磁共振（NMR）成像在神经病理学中的潜在应用。对22只成年雄性大鼠脑切片中冷冻损伤诱导的脑水肿进行成像，以观察其时间顺序。在另外12张脑切片图像中评估血脑屏障通透性变化。静脉注射乙二胺四乙酸二钠-锰溶液作为血脑屏障通透性的指标。通过改变核磁共振成像参数实现对比度增强。高分辨率成像能够可视化胼胝体，其厚度仅为0.2 - 0.4毫米。灰质中的水肿程度通过显著的对比清晰显示；水肿与周围正常皮质之间含水量存在细微差异时未观察到一致的结果。结果，清楚地观察到了脑水肿的时间顺序。从循环血液中通过受损毛细血管壁渗漏的锰-乙二胺四乙酸对核磁共振图像有“反常增强”效应；这种效应可能适用于评估核磁共振图像中的血脑屏障通透性变化。
