Wright R K, Buehler B A, Schott S N, Rennert O M
Pediatr Res. 1978 Aug;12(8):830-3. doi: 10.1203/00006450-197808000-00005.
Adenylate cyclase activity was measured in membrane preparations of cultured fibroblasts from controls and patients with cystic fibrosis. Enzyme activity increased as the transition from exponential growth to confluence occurred; sodium fluoride-stimulated activity more markedly displayed this relationship than basal cyclase activity. The in vitro addition of spermine (1 X 10(-6) to 2 X 10(-3) M) to membrane preparations caused inhibition of basal and sodium fluoride-stimulated enzyme activity, with 50% inhibition of basal activity occurring at 10(-6) M spermine. Spermidine (10(-4) M) caused 15--25% inhibition of adenylate cyclase activity. The increase in fibroblast adenylate cyclase activity during the transition from exponential growth was comparable in cells obtained from cystic fibrosis patients and control subjects. Basal and sodium-fluoride stimulated adenylate cyclase activity as well as inhibition of this enzyme activity by spermidine and spermine were undistinguishable between the different cell genotypes. A potential modulation of cellular proliferative activity through polyamine interaction with the adenylate cyclase system is postulated.
在来自对照者和囊性纤维化患者的培养成纤维细胞膜制剂中测量了腺苷酸环化酶活性。随着从指数生长向汇合状态的转变，酶活性增加；与基础环化酶活性相比，氟化钠刺激的活性更明显地呈现这种关系。向膜制剂中体外添加精胺（1×10⁻⁶至2×10⁻³M）会导致基础和氟化钠刺激的酶活性受到抑制，在10⁻⁶M精胺时基础活性受到50%的抑制。亚精胺（10⁻⁴M）导致腺苷酸环化酶活性受到15%至25%的抑制。从指数生长转变过程中，囊性纤维化患者和对照者的细胞中，成纤维细胞腺苷酸环化酶活性的增加是相当的。不同细胞基因型之间，基础和氟化钠刺激的腺苷酸环化酶活性以及亚精胺和精胺对该酶活性的抑制作用没有区别。推测通过多胺与腺苷酸环化酶系统的相互作用可能对细胞增殖活性进行调节。
