Gahl W A, Changus J E, Pitot H C
Pediatr Res. 1976 May;10(5):531-5. doi: 10.1203/00006450-197605000-00005.
The effects of spermidine and spermine at varying concentrations upon the replicative ability of human fibroblasts in cell culture have been studied. The average concentrations of spermidine causing a 50% inhibition of prolifertion (ID50) after 3 days of growth for three normal cell strains and three strains derived from patients with cystic fibrosis (CF) were 4.4 X 10(-6) +/- 1.2 M and 6.2 X 10(-6) +/- 2.1 M, respectively. The values for spermine were 2.0 X 10(-6) +/- 0.5 M for normal and 2.2 X 10(-6) +/- 0.1 M for fibroblasts from cystic fibrosis patients. No significant difference between the replicative ability of normal and CF cell strains was seen over a wide range of polyamine concentrations employed for a period of up to 3 days.
研究了不同浓度的亚精胺和精胺对细胞培养中人类成纤维细胞复制能力的影响。对于三种正常细胞株和三种源自囊性纤维化（CF）患者的细胞株，生长3天后导致增殖50%抑制（ID50）的亚精胺平均浓度分别为4.4×10⁻⁶±1.2 M和6.2×10⁻⁶±2.1 M。精胺的相应值，正常细胞为2.0×10⁻⁶±0.5 M，囊性纤维化患者的成纤维细胞为2.2×10⁻⁶±0.1 M。在长达3天的时间内，在所使用的广泛多胺浓度范围内，未观察到正常细胞株和CF细胞株的复制能力有显著差异。
