Weber E J
Biochem Genet. 1983 Feb;21(1-2):1-13. doi: 10.1007/BF02395387.
The percentage of linoleic acid in corn germ oil of three crosses, C103D x B73, C103D x B84, and T220 x H51, and their reciprocals was investigated. Corn germ oil from F2, F3, and backcrossed generations was also examined. More than one gene locus appeared to be involved in conditioning the linoleic acid content in these crosses. Strong maternal effects were exhibited in the F1's. Genotype also superimposed variations in fatty acid compositions within the characteristic lipid class patterns of the phospholipids, phosphatidylcholine, phosphatidylethanolamine, and phosphatidylinositol. Fatty acid placements in triglycerides, digalactosyldiglycerides, and phospholipids of one inbred, H51, were determined by lipase and phospholipase hydrolysis. The overall pattern of placement showed that the fatty acids at the 1 position were predominantly saturated saturated and those at the 2 position were predominantly unsaturated, but the fatty acid distribution was different for each individual lipid class. The molecular species of the phosphatidylcholines and phosphatidylethanolamines were separated by silver nitrate thin-layer chromatography. The major differences in the molecular species were a higher level of the dienoic-dienoic species and a lower level of the monoenoic-monoenoic species in the phosphatidylethanolamines than in the phosphatidylcholines.
对三个杂交组合C103D×B73、C103D×B84和T220×H51及其反交组合的玉米胚芽油中亚油酸的百分比进行了研究。还检测了F2、F3和回交世代的玉米胚芽油。在这些杂交组合中，似乎有一个以上的基因位点参与调节亚油酸含量。F1代表现出强烈的母体效应。基因型也在磷脂、磷脂酰胆碱、磷脂酰乙醇胺和磷脂酰肌醇的特征脂质类模式内叠加了脂肪酸组成的变化。通过脂肪酶和磷脂酶水解测定了一个自交系H51的甘油三酯、二半乳糖甘油二酯和磷脂中的脂肪酸位置。总体分布模式表明，1位的脂肪酸主要是饱和的，2位的脂肪酸主要是不饱和的，但每种脂质类别的脂肪酸分布不同。通过硝酸银薄层色谱法分离了磷脂酰胆碱和磷脂酰乙醇胺的分子种类。分子种类的主要差异在于，磷脂酰乙醇胺中双烯-双烯种类的水平高于磷脂酰胆碱，单烯-单烯种类的水平低于磷脂酰胆碱。
