Lin Y H, Yu C, Huang A H
Arch Biochem Biophys. 1986 Jan;244(1):346-56. doi: 10.1016/0003-9861(86)90123-2.
Lipases from several seed species were shown to be relatively specific on triacylglycerols containing the major fatty acid components of the storage triacylglycerols in the same species. In a direct comparison using individual triacylglycerol as well as mixed triacylglycerol preparations, highest activities were observed in corn lipase on trilinolein and triolein, castor bean lipase on triricinolein, rapeseed lipase on trierucin, and elm seed lipase on tricaprin. This pattern of fatty acyl specificity was also observed on diacylglycerols, monoacylglycerols, and fatty acyl 4-methylumbelliferone, although the pattern became less distinct. The seed lipases were inactive on lecithins. Corn lipase was more active on tri- than di- or monolinolein, and released linoleic acids from both primary and secondary positions. As judged from the kinetics of hydrolysis of rac-glyceryl-2,3-stearate-1-oleate and rac-glyceryl-1,3-stearate-2-oleate, and of trilinolein and dilinoleins, corn lipase exerted some degree of preference in releasing fatty acid from the primary than the secondary position of a triacylglycerol. At the primary position, corn lipase was more active on oleyl ester than stearyl ester.
研究表明，来自几种种子的脂肪酶对含有同一物种储存三酰甘油主要脂肪酸成分的三酰甘油具有相对特异性。在使用单个三酰甘油以及混合三酰甘油制剂的直接比较中，玉米脂肪酶对三油酸甘油酯和三油酸甘油酯、蓖麻籽脂肪酶对三蓖麻酸甘油酯、油菜籽脂肪酶对三芥酸甘油酯以及榆籽脂肪酶对三癸酸甘油酯的活性最高。在二酰甘油、单酰甘油和脂肪酰4-甲基伞形酮上也观察到这种脂肪酰特异性模式，尽管这种模式变得不那么明显。种子脂肪酶对卵磷脂无活性。玉米脂肪酶对三亚油酸甘油酯的活性高于二亚油酸甘油酯或单亚油酸甘油酯，并从一级和二级位置释放亚油酸。从外消旋甘油-2,3-硬脂酸酯-1-油酸酯和外消旋甘油-1,3-硬脂酸酯-2-油酸酯以及三油酸甘油酯和二油酸甘油酯的水解动力学判断，玉米脂肪酶在从三酰甘油的一级位置比二级位置释放脂肪酸时表现出一定程度的偏好。在一级位置，玉米脂肪酶对油酰酯的活性高于硬脂酰酯。
